Primer

Part:BBa_K4816004:Design

Designed by: Julia Alexander   Group: iGEM23_Bonn-Rheinbach   (2023-09-25)


nirS primer reverse


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Specific binding to nirS gene. Containing of restriction site for restriction digest and cloning into plasmid vector after PCR amplification.


Source

Sequence was designed on the basis of the DNA sequence for the nirS gene of Paracoccus denitrificans found on KEGG https://www.genome.jp/entry/pde:Pden_2487

References